Distribution, fauna and seasonal variation of sandflies, simultaneous detection of nuclear internal transcribed spacer ribosomal DNA gene of Leishmania major in Rhombomys opimus and Phlebotomus papatasi, in Natanz district in central part of Iran.
نویسندگان
چکیده
BACKGROUND Zoonotic cutaneous leishmaniasis (ZCL) due to Leishmania major is increasing in many parts of Iran. This disease originally is a disease found in gerbils. Leishmania parasites are transmitted by sandflies that live and breed in gerbil burrows. Nested PCR amplified Leishmania ITS1-5.8S rRNA gene in both main reservoir host "Rhombomys opimus" and in the "Phlebotomus papatasi" main vector of ZCL, in Iran. Population differentiation and seasonal variation of sandflies were analyzed at a microgeographical level in order to identify any isolation by distance, habitat or seasons. METHODS Populations of sandflies were sampled from the edges of villages in Natanz, Isfahan province, Iran, using the Centers for Disease Control traps and sticky papers. Individual sandflies were identified based on external and internal morphological characters. Nested PCR protocols were used to amplify Leishmania ITS1-5.8S rRNA gene, which were shown to be species-specific via DNA sequence. RESULTS A total of 4500 sandflies were collected and identified. P. papatasi, Phlebotomus sergenti and Phlebotomus jacusieli from genus Phlebotomus and Sergentomyia sintoni and Sergentomyia clydei from genus Sergentomyia were identified in this region. P. papatasi was the most abundant sandfly in the collections. Ten out of 549 female P. papatasi and four out of 19 R. opimus were found to be infected with L. major. CONCLUSION Seasonal activity of sandflies starts in June and ends in November. Abundance of P. papatasi was in September. Finding and molecular typing of L. major in P. papatasi and R. opimus confirmed the main vector and reservoir in this region.
منابع مشابه
Distribution, Fauna and Seasonal Variation of Sandflies, Simultaneous Detection of Nuclear Internal Transcribed Spacer Ribosomal DNA Gene of Leishmania major in Rhombomys opimus and Phlebotomus papatasi, in Natanz District in Central Part of Iran
Background: Zoonotic cutaneous leishmaniasis (ZCL) due to Leishmania major is increasing in many parts of Iran. This disease originally is a disease found in gerbils. Leishmania parasites are transmitted by sandflies that live and breed in gerbil burrows. Nested PCR amplified Leishmania ITS1-5.8S rRNA gene in both main reservoir host “Rhombomys opimus” and in the “Phlebotomus papatasi” main vec...
متن کاملMolecular Investigation of the Population Differentiation of Phlebotomus papatasi, Important Vector of L. major, in Different Habitats and Regions of Iran
Phlebotomus papatasi is an important vector of L. major in Iran. P. papatasi was collected from peridomestic animal shelters, inside and around the houses and also the nearby burrows of the gerbil reservoir hosts, Rhombomys opimus, in several provinces in Iran. Mitochondrial Cytochrome b (Cyt b) of sandflies, which is a maternally-inherited gene marker, was used to see if there is any "isolatio...
متن کاملMONTHLY VARIATION O F LEISHMANIA MAJOR MON-26 INFECTION RATES IN PHLEBOTOMUS PAPATASI (DIPTRA : PSYCHODIDAE) FROM RODENT BURROWS IN BADROOD AREA OF IRAN
Following an epidemiological survey of zoonotic cutaneous leishmaniosis (ZCL) in some villages of Badrood, a rural district north of the city of Natanz, central Iran, Phlebotomus (Phlebotomus ) papatasi Scopoli were found to be naturally infected with Leishmania (Leishmania) major zymodeme MON-26. Sandflies were collected and dissected biweekly from rodent burrows during sandfly season, Ap...
متن کاملMolecular Investigation of the Population Differentiation of Phlebotomus papatasi, Important Vector of L. major, in Different Habitats and Regions of Iran
Phlebotomus papatasi is an important vector of L. major in Iran. P. papatasi was collected from peridomestic animal shelters, inside and around the houses and also the nearby burrows of the gerbil reservoir hosts, Rhombomys opimus, in several provinces in Iran. Mitochondrial Cytochrome b (Cyt b) of sandflies, which is a maternally-inherited gene marker, was used to see if there is any "isolatio...
متن کاملSeasonal and Physiological Variations of Phlebotomus papatasi Salivary Gland Antigens in Central Iran
BACKGROUND Sand fly saliva helps parasite establishment and induce immune responses in vertebrate hosts. In the current study, we investigated the modulation of Phlebotomus papatasi salivary gland antigen expression by seasonal and biological factors. METHODS Sand flies were grouped according to physiological stages such as unfed, fed, semi-gravid, gravid, parous, nulliparous, infected or non...
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ورودعنوان ژورنال:
- Iranian biomedical journal
دوره 16 2 شماره
صفحات -
تاریخ انتشار 2012